《植物生理学报》 2010, 46(12): 1219-1224

苦马豆和披针叶黄华种子硬实特性与活力关系

王进¹, 张勇^2,*, 张有富¹, 颜霞¹, 孟嫣¹

河西学院1农学系, 2生物系, 甘肃张掖734000

通信作者：张勇；E-mail: zhangyong@hxu.edu.cn；Tel: 0936-8285056

摘　要：

将苦马豆和披针叶黄华种子在恒温 25 ℃下吸胀, 每 24 h 取出吸胀种子, 16 d 后未吸胀的种子为硬实种子(H), 硬实种 子用硫酸处理后恒温吸胀 24 h, 与非硬实种子进行发芽试验和各项活力指标测定。结果显示每日内吸胀的种子数量随时 间推移以一定比列下降, 苦马豆非硬实种子第 3 天后吸胀率下降到 1%, 第 13~16 天突然上升后又下降到 1%, 披针叶黄华非 硬实种子第 3 天后下降到 1%, 第 9、10 天突然上升后又下降到 1%。两种豆类都显示出硬实种子发芽率、发芽指数、活力 指数、脱氢酶活性、呼吸速率和超氧化物歧化酶(SOD)活性均高于非硬实种子, 而电导率、浸出液可溶性糖和丙二醛(MDA) 含量低于非硬实种子, 缓慢吸胀的硬实种子活力指标高于快速吸胀的硬实种子, 这表明硬实种子活力高于非硬实种子, 硬 实种子吸胀过程中存在吸胀损伤。而在非硬实种子中, 根据以上活力指标判断, 晚吸胀的种子比早吸胀的种子活力高。

关键词：苦马豆; 披针叶黄华; 硬实种子; 非硬实种子; 种子活力; 吸胀损伤

收稿：2010-07-23   修定：2010-09-10

资助：河西学院西部资源环境化学重点实验室项目(XZ0602)和河西学院硕士研究生导师科研项目(09002)。

Relationship between Hard Seed and Seed Vigor of Swainsonia salsula and Thermopsis lanceolate

WANG Jin¹, ZHANG Yong^2,*, ZHANG You-Fu¹, YAN Xia¹, MENG Yan¹

¹College of Agronomy,²College of Biology, Hexi University, Zhangye, Gansu 734000, China

Corresponding author: ZHANG Yong; E-mail: zhangyong@hxu.edu.cn; Tel: 0936-8285056

Abstract:

Seeds of Swainsonia salsula and Thermopsis lanceolate were imbibed at 25 ºC and were taken out as a sample every 24 h, after 16-day were treatment, the non-imbibing seeds were regard as hard seed (H). Treated with sulfate, the 24-hour imbibed hard seeds were subjected to germination and several vigor tests, with nonhard seed as control. The results indicated that the numbers of newly imbibed seeds were gradually decreased every day with soaking time delayed. Imbibition rate of S. salsula non-hard seed decreased to 1% after 3 days, and it increased little then deceased to 1% again in 13^th to 16^th day. Imbibition rate of T. lanceolate non-hard seed decreased to 1% after 3 days, and it increased little then deceased to 1% again in 9^th to 10^th day. The germination rate, germination index, vigor index, respiration rate, the activities of dehydrogenase and SOD of hard seeds were significantly higher than those of non-hard seeds in both species, while the relative conductivity, the contents of soluble sugar and malondialdehyde of hard seeds were lower than those of non-hard seeds. The vigor of seeds imbibed quickly was higher than that of seeds imbibed slowly, which showed that the vigor of hard seeds were higher than that of non-hard seeds, there was a soaking injury during imbibition process. The vigor of seeds imbibed later was higher than that of seeds imbibed earlier for non-hard seed basing on vigor indexes above.

Key words: Swainsonia salsula; Thermopsis lanceolate; hard seed; non-hard seed; seed vigor; soaking injury